Recombinant human PAG / Cbp is expressed in E. coli.DNA sequence encoding intracellular domain of human Cbp/PAG (amino acids 41- 432) was fused with a His tag at the N-terminus.
A calculated molecular mass of fussion protein is 45.3 kDa. As a result of highly acidic character of Cbp/PAG (predicted isoelectric point pI 4.4) and further modification by phosphorylation, the recombinant protein migrates as a 70 - 85 kDa protein in SDS-PAGE under reducing conditions.
